In Vitro Optical Imaging for Regenerative Medicine
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Introduction
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| Regenerative medicine is an emerging, interdisciplinary field,
the goal of which is to aid the body in regenerating diseased
or damaged tissues and organs. To date, the promise of commercially
available tissue engineered products (TEMPs) has been largely
unrealized. This is due in part to the high cost and long times
required to bring the products to market.
The time and cost of developing TEMPs is driven in large
measure by complex interactions between materials and biological
systems, and the general inability to acquire sufficiently
detailed information regarding these interactions in a rapid
and non-invasive way.
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Objective
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Our approach is to develop in vitro optical imaging techniques
coupled with data reduction methods that will result in metrics
that reflect the response of cells to the scaffold environment.
We are currently focusing on two techniques:
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Broadband coherent anti-stokes Raman spectroscopy (CARS) is
high resolution chemical microscopy that monitors cell activity
and growth along with local scaffold morphology and relevant
surface and solution chemical components.
Collinear optical coherence and confocal fluorescence microscopies
correlate cell activity with scaffold microstructure.
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NIST Role
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NIST's mission is to develop and promote measurements, standards,
and technology to enhance productivity, facilitate trade,
and improve the quality of life.
This project is focused on improving the safety and enhancing
the efficacy of materials used in regenerative medicine through
reference materials and advanced metrologies.
NIST leadership will enable open collaboration and technology
transfer within the tissue engineering community.
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Highlights
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Left – Broadband CARS image of a polymer blend consisting
of polystyrene (green), polymethyl methacrylate (red), and polyethylene
terephthalate (yellow). Each component is “chemically”
identified with its unique broadband CARS spectrum.
Right – One type of data reduction method: Qualitative
interpretation of collinear OCM/CFM data using immersive visualization.
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Customers and Impact
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Tissue engineering community
Biomaterials community
Broader materials science community
Microscopes provide new view for tissue engineering
10 Dec 2003 |
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OCM/CFM:
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Joy Dunkers*
Forrest Landis
Jean Stephens
Martin Chiang
Xianfeng Wang
Judy Devaney (ITL)*
John Hagedorn (ITL)
Steve Satterfield (ITL)
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CARS:
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Marcus Cicerone*
Tak Kee
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