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Gradient Library Screening of Cell-Material Interactions
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Introduction
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| Gradient libraries have been applied to determine
the functional relationship between material parameters and
cellular responses. These gradients could be applied as reference
materials to validate materials or processing methods in the
fabrication of tissue engineered medical products. They could
also be used as test patterns to assess the viability of cells
for tissue engineering applications by comparing the pattern
of cell responses along the gradient with reference cell responses.
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Experimental Approach
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Gradients in cell adhesion strength have been
prepared by synthesizing grafts of poly(hydroxyethylmethacrylate)
(PHEMA) on silicon wafers via atom transfer radical polymerization.
Surfaces grafted with PHEMA at high density resist protein and
cell adsorption. At low grafting density, PHEMA partially blocks
protein adsorption and reduces the density of adhesion proteins
available for cell receptors.
Cell adhesion strength was assessed by measuring the density
of adherent cells and cell area as a function of substrate properties.
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Results
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| Cell adhesion strength was assessed by measuring
the density of adherent cells and cell area as a function
of substrate properties.
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| The density of adherent cells tracks the water contact angle
of the gradient library. A non-monotonic dependence was observed
for both quantities.
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Future Activities
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Measure PHEMA graft density.
Measure conformation of adherent fibronectin.
Develop single-cell statistics as measurement tool.
Extend tests to other cells types.
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Publications
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Mei et al. JACS (2004)
Mei et al. In preparation.
Presented at 2004 World Biomaterials Congress
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NIST Contributors:
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Ying Mei
Matt Becker
Newell Washburn
Tao Wu
Kathryn Beers
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Collaborators:
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John Elliott (CSTL)
Kurt Langenbach (CSTL) |
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